Core Research Facility Technician III - NextGen DNA Sequencing

Core Research Facility Technician III - NextGen DNA Sequencing

Job No: 531186
Work Type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Biology/Life Science, Grant or Research Administration
Department: 11600107 - RE-ICBR-NEXTGEN

Job Description


Classification Title:

Core Research Facility Technician III

Job Description:

-Technical Functions
Plan and execute Illumina MiSeq, iSeq, NovaSeq and ONT sequencing runs.
Maintain and troubleshoot Illumina MiSeq, iSeq, NovaSeqand ONTinstruments.
Evaluate MiSeq, iSeq, NovaSeqand ONT instruments data and prepare written reports for research projects.
Construct and perform quality assessment of samples, and sequence libraries for the ONT, PacBio and Illumina platforms.
Test, troubleshoot and implement new protocols for NGS library construction protocol, using novel procedures as they become available in the literature or from vendors.
Perform all basic QC assays that are part of sample preparation processes for NGS, including, but not limited to: Fluorometric assays (on the QUBIT and FLUOstar Omega BMG), size selection protocol (on the SAGE-ELF and gels, magnetic bead purification (AMPure), and DNA fragmentation protocols (Covaris S220 and Covaris E220).
Document all work by using established lab tracking sheets (LTS) and digital documentation tools (FileShare).
Communicate with supervisor and other core personnel about experimental results in a timely and professional manner. Work with supervisor to define deliverables and timelines for project completion.
Contribute to the preparation, updating and organization of Standard Operating Procedures (SOPs).

-General Lab Operation and Maintenance Functions
Evaluate new laboratory methods for the implementation of new services in the NGS core.
Contribute to the development and maintenance of lab tracking system (LIMS) in the NGS workflows.
Attend all relevant coordination and organizational meetings as requested by supervisor.
Contribute to the maintenance of a complete inventory of reagents, consumables and equipment used for NGS sequencing.
Assist lab manager in keeping track of inventories and placing orders of reagents, consumables for ONT, PacBio and Illumina sequencing as needed.
Assist the Environmental Health and Safety (EHS) manager for the NextGen lab as needed, for the purpose of complying with all EHS institutional requirements.

- Educational Functions
Keep abreast of new developments in the NGS field through the literature and scientific meetings.
Help in the training of other personnel in the NGS Core group.
Participate in cross-training activities in the NGS core and ICBR as necessary or as requested by lab director.

- Misc.
Help in laboratory organization, cleaning and general maintenance
Receive, store, and help document orders arriving into the lab. Help send off outgoing parcels when requested.
Other duties as assigned

Expected Salary:

$40,000 - $42,500, commensurate with education and experience

Minimum Requirements:

BA/BS in related scientific field required with hands-on experience in basic laboratory and scientific methods. Optionally, 4 years of experience in science related field with at least 3 years in core specific applications. Research-based experience preferred.
Core or technology specific licensure and/or certifications where applicable. Additionally yearly certificate and compliance training if required.

Preferred Qualifications:

The preferred applicant will have a familiarity with the basic techniques and instrumentation used in the molecular biology laboratory, including specific NGS procedures that include (but are not limited to):

Genomic DNA purification, quantification and manipulation, PCR, NGS DNA library construction and quality assessment/evaluation.

Use of robotics for high throughput processing of moeluclar biology samples.

Hands-on experience with Illumina, PacBio and/or ONT NGS instrumentation and technology.

Basic laboratory citizenship including organization of equipment and supply inventory,

Instrument run coordination and maintenance.

Preparation of common buffers and solutions.

Computer literacy in Windows/UNIX systems, Microsoft office package.

Standard Operating Procedures (SOPs), iLAB and laboratory information management system (LIMS).

Special Instructions to Applicants:

In order to be considered, you must upload your cover letter and resume.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:
No



Applications Close: 01 May 2024

To apply, visit

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